The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Detects a band of approximately 200 kDa (predicted molecular weight: 176 kDa). Do not denature WB protein lysate, denaturing results in loss of Nestin specific immunoreactivity (See Abreview).
Is unsuitable for ICC/IF or IHC-P.
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
CNS stem cells.
Belongs to the intermediate filament family.
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France
A slightly higher band obtained than expected, but as predicted in the datasheet. Denaturing of the WB lysate results in a loss of Nestin specific immunoreactivity. NB: Equal protein loading was verified.