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Our Abpromise guarantee covers the use of ab7659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Detects a band of approximately 200 kDa (predicted molecular weight: 176 kDa). Do not denature WB protein lysate, denaturing results in loss of Nestin specific immunoreactivity (See Abreview).|
This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, FranceA slightly higher band obtained than expected, but as predicted in the datasheet. Denaturing of the WB lysate results in a loss of Nestin specific immunoreactivity. NB: Equal protein loading was verified.