The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
CNS stem cells.
Belongs to the intermediate filament family.
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
ab76684, at a 1/50 dilution, staining Nestin in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry. A peroxidase conjugated secondary antibody was then used, followed by DAB staining.