概述

  • 产品名称Anti-Nestin抗体
    参阅全部 Nestin 一抗
  • 描述
    兔多克隆抗体to Nestin
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 325 and 375 of human Nestin (NP_006608.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells

性能

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应用

Our Abpromise guarantee covers the use of ab85684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 5 - 10 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 177 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • 组织特异性CNS stem cells.
    • 序列相似性Belongs to the intermediate filament family.
    • 发展阶段Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • 翻译后修饰Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Anti-Nestin antibody 图像

    • All lanes : Anti-Nestin antibody (ab85684) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 177 kDa


      Exposure time : 3 minutes
    • Nestin was immunoprecipitated using ab85684 at 10 µg/mg of whole cell lysate from HeLa cells.
      20% of the IP was loaded, and probed with ab85684 at 0.4 µg/ml.
      Detection: chemiluminescence with exposure time of 30 seconds.

    Anti-Nestin antibody (ab85684)参考文献

    ab85684 has not yet been referenced specifically in any publications.

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