Anti-Nestin抗体[2C1.3A11] (ab18102)
Key features and details
- Mouse monoclonal [2C1.3A11] to Nestin
- Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-Nestin抗体[2C1.3A11]
参阅全部 Nestin 一抗 -
描述
小鼠单克隆抗体[2C1.3A11] to Nestin -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WB, Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Human
不与反应: Rat -
免疫原
A 150 amino acid fragment from the cloned human Nestin.
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常规说明
This highly specific antibody to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
Primary antibody说明
This highly specific antibody to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. -
克隆
单克隆 -
克隆编号
2C1.3A11 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18102于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100 - 1/500.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 177 kDa.
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Flow Cyt | (1) |
1/200.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P |
Use at an assay dependent concentration.
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说明 |
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ICC/IF
1/100 - 1/500. |
WB
1/1000 - 1/5000. Predicted molecular weight: 177 kDa. |
Flow Cyt
1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. -
组织特异性
CNS stem cells. -
序列相似性
Belongs to the intermediate filament family. -
发展阶段
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. -
翻译后修饰
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
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数据库链接
- Entrez Gene: 10763 Human
- Omim: 600915 Human
- SwissProt: P48681 Human
- Unigene: 527971 Human
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别名
- ESTM 46 antibody
- FLJ 21841 antibody
- FLJ21841 antibody
see all
图片
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All lanes : Anti-Nestin antibody [2C1.3A11] (ab18102) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : NES knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 177 kDaLanes 1 - 2: Merged signal (red and green). Green - ab18102 observed at 300 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab18102 was shown to recognize NES in wild-type HAP1 cells as signal was lost at the expected MW in NES knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NES knockout samples were subjected to SDS-PAGE. Ab18102 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunofluorescence staining of U251 cells (glioblastoma cell line) stained using ab18102(green) and bisbenzimide (blue)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of migrating tumor cells labelling Nestin with ab18102 at 2µg/ml. Cytoplasm and membrane positive staining shown.
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All lanes : Anti-Nestin antibody [2C1.3A11] (ab18102) at 1/1000 dilution
Lane 1 : Total rat brain protein at 5 µg
Lane 2 : Human CNS progenitor cell protein at 1 µg
Lane 3 : Human CNS progenitor cell protein at 5 µg
Predicted band size: 177 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of xenograft tumor cells labelling Nestin with ab18102.
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Overlay histogram showing SH-SY5Y cells stained with ab18102 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18102, 1/200 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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Immunohistochemistry of Anti-Nestin on formalin fixed paraffin embedded prostate nerve tissue using ab18102
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (22)
ab18102 被引用在 22 文献中.
- Song S et al. Low-intensity pulsed ultrasound-generated singlet oxygen induces telomere damage leading to glioma stem cell awakening from quiescence. iScience 25:103558 (2022). PubMed: 34988401
- Pushchina EV et al. Molecular Markers of Adult Neurogenesis in the Telencephalon and Tectum of Rainbow Trout, Oncorhynchus mykiss. Int J Mol Sci 23:N/A (2022). PubMed: 35163116
- Hou D et al. Circular RNA circASPM promotes the progression of glioblastoma by acting as a competing endogenous RNA to regulate miR-130b-3p/E2F1 axis. J Cancer 13:1664-1678 (2022). PubMed: 35371308
- Chen K et al. LRP1 is a neuronal receptor for α-synuclein uptake and spread. Mol Neurodegener 17:57 (2022). PubMed: 36056345
- Wang Z et al. Hypoxia-induced PLOD1 overexpression contributes to the malignant phenotype of glioblastoma via NF-?B signaling. Oncogene N/A:N/A (2021). PubMed: 33420370