概述

  • 产品名称
    Anti-Nesprin3抗体[Nsp3]
    参阅全部 Nesprin3 一抗
  • 描述
    小鼠单克隆抗体[Nsp3] to Nesprin3
  • 经测试应用
    适用于: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
    不与反应: Human, Zebrafish
  • 免疫原

    Fusion protein encoding the seventh spectrin repeat of Nesprin3 and a proprietary protein.

  • 阳性对照
    • Mouse embryonic fibroblasts.

性能

应用

Our Abpromise guarantee covers the use of ab123031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 122 kDa. FITC-conjugated secondary antibodies or avidin-biotinylated horseradish peroxidase complex (ABC) may be used as detection reagents.
IHC-P 1/100 - 1/1000.
IHC-Fr 1/100 - 1/1000. fixed with methanol
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
  • 序列相似性
    Belongs to the nesprin family.
    Contains 1 KASH domain.
    Contains 2 spectrin repeats.
  • 结构域
    The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
  • 细胞定位
    Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C14orf49 antibody
    • Chromosome 14 open reading frame 49 antibody
    • FLJ25605 antibody
    • MGC75267 antibody
    • Nesp3 antibody
    • Nesprin 3 antibody
    • Nesprin-3 antibody
    • NET53 antibody
    • Nuclear Envelope Spectrin Repeat Protein 3 antibody
    • Spectrin Repeat Containing, Nuclear Envelope Family Member 3 antibody
    • SYNE3 antibody
    • SYNE3_HUMAN antibody
    see all

图片

  • ab123031 staining Nesprin3 in cultured mouse embryonic fibroblast by Immunofluorescence (green). The nuclear DNA is counterstained with DAPI (blue).

文献

This product has been referenced in:
  • Alam SG  et al. The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity. Sci Rep 6:38063 (2016). ICC/IF . Read more (PubMed: 27905489) »
  • Gutiérrez-Fernández A  et al. Loss of MT1-MMP causes cell senescence and nuclear defects which can be reversed by retinoic acid. EMBO J 34:1875-88 (2015). Read more (PubMed: 25991604) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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