概述

  • 产品名称Anti-Nesprin3抗体
    参阅全部 Nesprin3 一抗
  • 描述
    兔多克隆抗体to Nesprin3
  • 特异性This antibody detects endogenous levels of total Nesprin3 protein.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthesized peptide derived from an internal region of human Nesprin3.

  • 阳性对照
    • Extracts from Jurkat cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitopespecific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab74261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 112 kDa.
ELISA 1/40000.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
  • 序列相似性Belongs to the nesprin family.
    Contains 1 KASH domain.
    Contains 2 spectrin repeats.
  • 结构域The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
  • 细胞定位Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C14orf49 antibody
    • Chromosome 14 open reading frame 49 antibody
    • FLJ25605 antibody
    • MGC75267 antibody
    • Nesp3 antibody
    • Nesprin 3 antibody
    • Nesprin-3 antibody
    • NET53 antibody
    • Nuclear Envelope Spectrin Repeat Protein 3 antibody
    • Spectrin Repeat Containing, Nuclear Envelope Family Member 3 antibody
    • SYNE3 antibody
    • SYNE3_HUMAN antibody
    see all

Anti-Nesprin3 antibody 图像

  • All lanes : Anti-Nesprin3 antibody (ab74261) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 112 kDa
    Observed band size : 112 kDa
  • ICC/IF image of ab74261 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74261, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Nesprin3 antibody (ab74261)参考文献

This product has been referenced in:
  • Coffinier C  et al. Abnormal development of the cerebral cortex and cerebellum in the setting of lamin B2 deficiency. Proc Natl Acad Sci U S A 107:5076-81 (2010). WB, ICC/IF ; Mouse . Read more (PubMed: 20145110) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab74261.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"