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There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus.These proteins are called Nesprin l and 2 for nuclear envelope spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM-domain-containing sequence essential for perinuclear localization. Nesprin l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as "dystrophins of the nucleus" to maintain nuclear organization and structural integrity.
Our Abpromise guarantee covers the use of ab5250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC (PFA fixed)||Use at an assay dependent concentration. PubMed: 17159980Use at an assay dependent dilution. Perfused with 4% PFA in PBS from (PubMed:17159980).|
|WB||1/500 - 1/750. Detects a band of approximately 112 kDa.|
|ICC/IF||1/25 - 1/50. Fix cells with cold methanol: acetone 1: 1 for 10 minutes on ice.|
|IHC-Fr||1/25 - 1/50. Fix with cold methanol: acetone 1: 1 for 10 minutes on ice.|