Anti-Nephrocystin 4抗体(ab110670)

概述

  • 产品名称Anti-Nephrocystin 4抗体
    参阅全部 Nephrocystin 4 一抗
  • 描述
    兔多克隆抗体to Nephrocystin 4
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1350 to the C-terminus of Human Nephrocystin 4.

  • 阳性对照
    • This antibody gave a positive signal in HEK293 and HepG2 whole cell lysates. This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab110670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB 1/250. Detects a band of approximately 157 kDa (predicted molecular weight: 157 kDa).

靶标

  • 组织特异性Expressed in kidney, skeletal muscle, heart and liver, and to a lesser extent in brain and lung.
  • 疾病相关Defects in NPHP4 are the cause of nephronophthisis type 4 (NPHP4) [MIM:606966]; also known as familial juvenile nephronophthisis 4. NPHP4 is an autosomal recessive inherited disease resulting in end-stage renal disease at age ranging between 6 and 35 years. It is a progressive tubulo-interstitial kidney disorder characterized by polydipsia, polyuria, anemia and growth retardation. The most prominent histological features are modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts.
    Defects in NPHP4 are the cause of Senior-Loken syndrome type 4 (SLSN4) [MIM:606996]. SLSN is a renal-retinal disorder characterized by progressive wasting of the filtering unit of the kidney, with or without medullary cystic renal disease, and progressive eye disease. Typically this disorder becomes apparent during the first year of life.
  • 序列相似性Belongs to the NPHP4 family.
  • 细胞定位Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0673 antibody
    • Nephrocystin-4 antibody
    • nephronophthisis 4 antibody
    • Nephroretinin antibody
    • NPHP4 antibody
    • NPHP4_HUMAN antibody
    • POC10 antibody
    • POC10 centriolar protein homolog antibody
    • SLSN4 antibody
    see all

Anti-Nephrocystin 4 antibody 图像

  • ICC/IF image of ab110670 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110670 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Nephrocystin 4 antibody (ab110670) at 1/250 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 157 kDa
    Observed band size : 157 kDa
    Additional bands at : 100 kDa,117 kDa,200 kDa,31 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

Anti-Nephrocystin 4 antibody (ab110670)参考文献

ab110670 has not yet been referenced specifically in any publications.

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