The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Specifically expressed in podocytes of kidney glomeruli.
Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
Belongs to the immunoglobulin superfamily. Contains 1 fibronectin type-III domain. Contains 8 Ig-like C2-type (immunoglobulin-like) domains.
In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.
Phosphorylated on tyrosine residues.
Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
Western blot - Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298)
All lanes : Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298) at 1/50000 dilution
Lane 1 : HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin expression vector, whole cell lysate Lane 2 : HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate Lane 3 : HEK293T (human embryonic kidney epithelial cell) transfected with Y1217F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 138 kDa Observed band size : 138 kDa