Anti-Neomycin Phosphotransferase 2抗体(ab49975)

概述

  • 产品名称
    Anti-Neomycin Phosphotransferase 2抗体
    参阅全部 Neomycin Phosphotransferase 2 一抗
  • 描述
    兔多克隆抗体to Neomycin Phosphotransferase 2
  • 特异性
    ab49975 recognizes bacterial Neomycin Phosphotransferase 2 expressed in transgenic tobacco plants.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Synthetic peptide:

    AIPVSECSVQQWTTHAGL

    conjugated to KLH, corresponding to internal sequence amino acids 133-150 of Escherichia coli Neomycin Phosphotransferase 2

  • 阳性对照
    • Cytosolic fraction from the leaves of transgenic Nicotiana tabacum expressing E. coli Neomycin Phosphotransferase 2.

性能

应用

Our Abpromise guarantee covers the use of ab49975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 29 kDa.

靶标

  • 相关性
    Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
  • 数据库链接
    • 别名
      • Aminoglycoside 3' phosphotransferase antibody
      • APH(3') II antibody
      • APH(3')II antibody
      • aphA antibody
      • EC 2.7.1.95 antibody
      • Kan antibody
      • Kanamycin kinase type II antibody
      • Kanamycin kinase, type VIII antibody
      • kanamycin resistance gene antibody
      • Neo antibody
      • Neomycin kanamycin phosphotransferase type II antibody
      • Neomycin kanamycin phosphotransferase type VIII antibody
      • Neomycin phosphotransferase II antibody
      • Neomycine phosphotransferase antibody
      • NPT II antibody
      • NPTII antibody
      see all

    图片

    • Anti-Neomycin Phosphotransferase 2 antibody (ab49975) at 1/500 dilution + Whole cell lysate prepared from SW48 cancer cell line at 20 µg

      Secondary
      Goat anti-rabbit conjugated to HRP at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 29 kDa
      Observed band size : 29 kDa


      Exposure time : 5 minutes

      Image courtesy of an anonymous Abreview.

      See Abreview

    实验方案

    文献

    ab49975 has not yet been referenced specifically in any publications.

    客户评价及客户问答



    Unfortunately, I can confirm that if you are amplifying the Neo gene from pAcGFP N1 sequence you sent me, there is no homology with the immunogen used to raise the ab49975 antibody, so we would not expect to cross react with the expressed pro...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Escherichia coli Cell lysate - whole cell (SW48 cancer cell line)
    Loading amount
    20 µg
    Specification
    SW48 cancer cell line
    Gel Running Conditions
    Reduced Denaturing (gradient 4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 22 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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