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概述

  • 产品名称Anti-NENF抗体
    参阅全部 NENF 一抗
  • 描述
    兔多克隆抗体to NENF
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human) of 15 amino acids from near the C-terminus of NENF. (NP_037481).

  • 阳性对照
    • Human kidney tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab74474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 19 kDa).
ICC/IF Use a concentration of 20 µg/ml.

靶标

  • 功能Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme.
  • 序列相似性Belongs to the cytochrome b5 family. MAPR subfamily.
    Contains 1 cytochrome b5 heme-binding domain.
  • 结构域The cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Cell growth inhibiting protein 47 antibody
    • Cell immortalization-related protein 2 antibody
    • CIR2 antibody
    • Nenf antibody
    • NENF_HUMAN antibody
    • Neudesin antibody
    • Neuron derived neurotrophic factor antibody
    • Neuron-derived neurotrophic factor antibody
    • SCIRP10 antibody
    • SCIRP10 related protein antibody
    • Secreted protein of unknown function antibody
    • Spinal cord injury related protein 10 antibody
    • SPUF antibody
    • SPUF protein antibody
    see all

Anti-NENF antibody 图像

  • Immunocytochemistry/ Immunofluorescence - Anti-NENF antibody (ab74474)
    Immunocytochemistry/ Immunofluorescence - Anti-NENF antibody (ab74474)

    Immunofluorescence of NENF in rat kidney tissue with ab74474 at 20 ug/mL.

  • Western blot - NENF antibody (ab74474)
    Western blot - NENF antibody (ab74474)
    Lane 1 : Anti-NENF antibody (ab74474) at 1 µg/ml
    Lane 2 : Anti-NENF antibody (ab74474) at 2 µg/ml

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human kidney tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 19 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NENF antibody (ab74474)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NENF antibody (ab74474)

    ab74474 at 10 µg/ml staining NENF in rat kidney tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

Anti-NENF antibody (ab74474)参考文献

This product has been referenced in:
  • Stefanska B  et al. Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets. Clin Cancer Res 20:3118-32 (2014). WB ; Human . Read more (PubMed: 24763612) »

See 1 Publication for this product

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