- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-NENF抗体
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描述兔多克隆抗体to NENF
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宿主Rabbit
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经测试应用适用于: WB, ICC/IFmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic peptide (Human) of 15 amino acids from near the C-terminus of NENF. (NP_037481).
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阳性对照
- Human kidney tissue lysate
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab74474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 19 kDa). | |
| ICC/IF | Use a concentration of 20 µg/ml. |
靶标
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功能Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme.
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序列相似性Belongs to the cytochrome b5 family. MAPR subfamily.
Contains 1 cytochrome b5 heme-binding domain. -
结构域The cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82.
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细胞定位Secreted > extracellular space.
- Information by UniProt
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数据库链接
- Entrez Gene: 29937 Human
- Entrez Gene: 66208 Mouse
- Entrez Gene: 289380 Rat
- Omim: 611874 Human
- SwissProt: Q9UMX5 Human
- SwissProt: Q9CQ45 Mouse
- SwissProt: Q6IUR5 Rat
- Unigene: 461787 Human
see all -
别名
- Cell growth inhibiting protein 47 antibody
- Cell immortalization-related protein 2 antibody
- CIR2 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-NENF antibody (ab74474)Immunofluorescence of NENF in rat kidney tissue with ab74474 at 20 ug/mL.
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Western blot - Anti-NENF antibody (ab74474)Lane 1 : Anti-NENF antibody (ab74474) at 1 µg/ml
Lane 2 : Anti-NENF antibody (ab74474) at 2 µg/ml
All lanes : Human kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 19 kDa
Observed band size: 37 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NENF antibody (ab74474)ab74474 at 10 µg/ml staining NENF in rat kidney tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
文献
This product has been referenced in:
- Stefanska B et al. Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets. Clin Cancer Res 20:3118-32 (2014). WB ; Human . Read more (PubMed: 24763612) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"