The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Couples NMDA receptor signaling to the nucleus. Influences outgrowth of olfactory axons and migration of LHRH neurons.
组织特异性Highly expressed in adult and fetal brain, testis and kidney.
疾病相关Defects in NELF may be associated with idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
细胞定位Cell membrane. Nucleus. Found on the outside of the LHRH cell membrane and axons projecting from the olfactory pit and epithelium. Myristoylation is a prerequisite for extranuclear localization.