The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Couples NMDA receptor signaling to the nucleus. Influences outgrowth of olfactory axons and migration of LHRH neurons.
Highly expressed in adult and fetal brain, testis and kidney.
Defects in NELF may be associated with idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
Cell membrane. Nucleus. Found on the outside of the LHRH cell membrane and axons projecting from the olfactory pit and epithelium. Myristoylation is a prerequisite for extranuclear localization.