Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Orangutan
A region between residue 1 and 50 of human NEK7 (NP_598001.1)
HeLa and 293T cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab95873 was affinity purified using an epitope specific to NEK7 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 35 kDa.
Use at 5-15 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Information by UniProt
EC 22.214.171.124 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK7 antibody (ab95873)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling NEK7 with ab95873 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-NEK7 antibody (ab95873)
All lanes : Anti-NEK7 antibody (ab95873) at 0.4 µg/ml Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg Predicted band size: 35 kDa
Immunoprecipitation - Anti-NEK7 antibody (ab95873)
Detection of NEK7 by Western Blot of Immunprecipitate.
(1) ab95873 at 0.4µg/ml staining NEK7 which had been immunoprecipitated from HeLa whole cell lysate using ab95873 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), (2) control IgG.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-NEK7 antibody (ab95873) at 1 µg/ml Lane 1 : IP: ab95873 at 10µg/mg lysate Lane 2 : IP: Controle IgG at 10µg/mg lysate
has not yet been referenced specifically in any publications.
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