Full length protein, corresponding to amino acids 1-302 of Human NEK7
NEK7 transfected mammalian cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa).
Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Information by UniProt
EC 220.127.116.11 antibody
Western blot - NEK7 antibody (ab68060)
All lanes :
Anti-NEK7 antibody (ab68060) at 1/500 dilution
Lane 1 :
NEK7 transfected 293T cell lysate
Lane 2 :
Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary All lanes :
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size:
Observed band size:
28 kDa (
why is the actual band size different from the predicted?
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