概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to NEK7
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-302 of Human NEK7

  • 阳性对照
    • NEK7 transfected mammalian cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab68060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa).

靶标

  • 组织特异性
    Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
  • 序列相似性
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.7.11.1 antibody
    • NEK7 antibody
    • NEK7_HUMAN antibody
    • Never in mitosis A-related kinase 7 antibody
    • Never in mitosis gene a-related kinase 7 antibody
    • NIMA (never in mitosis gene a) related kinase 7 antibody
    • NIMA (never in mitosis gene a)-related expressed kinase 7 antibody
    • NimA related protein kinase 7 antibody
    • NIMA-related kinase 7 antibody
    • NimA-related protein kinase 7 antibody
    • Serine/threonine-protein kinase Nek7 antibody
    see all

Anti-NEK7 antibody 图像

  • All lanes : Anti-NEK7 antibody (ab68060) at 1/500 dilution

    Lane 1 : NEK7 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 34 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-NEK7 antibody (ab68060)参考文献

This product has been referenced in:
  • de Souza EE  et al. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res 13:4074-90 (2014). Read more (PubMed: 25093993) »

See 1 Publication for this product

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