概述

  • 产品名称Anti-NEK5抗体
    参阅全部 NEK5 一抗
  • 描述
    小鼠单克隆抗体to NEK5
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 601-708 of Human NEK5 with a proprietary tag.

  • 阳性对照
    • IMR-32 cell lysate; Recombinant tagged human NEK5 fragment.

性能

应用

Our Abpromise guarantee covers the use of ab77451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 81 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 序列相似性Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC75495 antibody
    • NEK5 antibody
    • NEK5_HUMAN antibody
    • Never in mitosis A-related kinase 5 antibody
    • NIMA (never in mitosis gene a) related kinase 5 antibody
    • NIMA related kinase 5 antibody
    • NimA related protein kinase 5 antibody
    • NimA-related protein kinase 5 antibody
    • OTTHUMP00000040896 antibody
    • Serine/threonine protein kinase Nek5 antibody
    • Serine/threonine-protein kinase Nek5 antibody
    see all

Anti-NEK5 antibody 图像

  • Anti-NEK5 antibody (ab77451) at 1 µg/ml + Recombinant tagged human NEK5 fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 81 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-NEK5 antibody (ab77451) at 1 µg/ml + IMR-32 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 81 kDa
    Observed band size : 81 kDa
    Additional bands at : 105 kDa. We are unsure as to the identity of these extra bands.

Anti-NEK5 antibody (ab77451)参考文献

ab77451 has not yet been referenced specifically in any publications.

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