概述

  • 产品名称Anti-NEK4抗体
    参阅全部 NEK4 一抗
  • 描述
    兔多克隆抗体to NEK4
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 350-400 (KQDLSNTTEL ATISSVNIDI LPAKGRDSVS DGFVQENQPR YLDASNELGG I) of Human NEK4 (NP_003148.2).

  • 阳性对照
    • HeLa whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab95189 was affinity purified using an epitope specific to NEK4 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab95189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 相关性NEK4 (NIMA related kinase 4) is a serine/threonine protein kinase related to the asperigillus NIMA (never in mitosis gene a) kinase. NEK4 is proposed to play a role in cell division. Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas. There are two named isoforms produced by alternative splicing.
    • 细胞定位Nuclear
    • 数据库链接
    • 别名
      • EC 2.7.11.1 antibody
      • MGC33171 antibody
      • Never in mitosis A related kinase 4 antibody
      • Never in mitosis gene A related kinase 4 antibody
      • NIMA (never in mitosis gene a) related kinase 4 antibody
      • NIMA (never in mitosis gene a)-related expressed kinase 4 antibody
      • NIMA related kinase 4 antibody
      • NimA related protein kinase 4 antibody
      • NLK2 antibody
      • NRK2 antibody
      • OTTHUMP00000213582 antibody
      • Pp12301 antibody
      • Serine/threonine kinase 2 antibody
      • Serine/threonine protein kinase 2 antibody
      • Serine/threonine protein kinase Nek4 antibody
      • Serine/threonine protein kinase NRK2 antibody
      • STK2 antibody
      see all

    Anti-NEK4 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NEK4 with ab95189 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of NEK4 in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab95189 at 3 µg/mg lysate for IP. An anti-NEK4 antibody, which recognizes a downstream epitope, was used at 1µg/ml for the subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.

    Anti-NEK4 antibody (ab95189)参考文献

    ab95189 has not yet been referenced specifically in any publications.

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