概述

  • 产品名称Anti-NEK4抗体
    参阅全部 NEK4 一抗
  • 描述
    兔多克隆抗体to NEK4
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 475-525 of human NEK4 (NP_003148.2)

  • 阳性对照
    • HeLa cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab95176 was affinity purified using an epitope specific to NEK4 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab95176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 95 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性NEK4 (NIMA related kinase 4) is a serine/threonine protein kinase related to the asperigillus NIMA (never in mitosis gene a) kinase. NEK4 is proposed to play a role in cell division. Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas. There are two named isoforms produced by alternative splicing.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • EC 2.7.11.1 antibody
    • MGC33171 antibody
    • Never in mitosis A related kinase 4 antibody
    • Never in mitosis gene A related kinase 4 antibody
    • NIMA (never in mitosis gene a) related kinase 4 antibody
    • NIMA (never in mitosis gene a)-related expressed kinase 4 antibody
    • NIMA related kinase 4 antibody
    • NimA related protein kinase 4 antibody
    • NLK2 antibody
    • NRK2 antibody
    • OTTHUMP00000213582 antibody
    • Pp12301 antibody
    • Serine/threonine kinase 2 antibody
    • Serine/threonine protein kinase 2 antibody
    • Serine/threonine protein kinase Nek4 antibody
    • Serine/threonine protein kinase NRK2 antibody
    • STK2 antibody
    see all

Anti-NEK4 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NEK4 with ab95176 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NEK4 antibody (ab95176) at 0.04 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg/ml
    Lane 2 : HeLa cell lysate at 15 µg/ml
    Lane 3 : HeLa cell lysate at 5 µg/ml


    Predicted band size : 95 kDa
    Detection: Chemiluminescence with exposure times of 3 minutes.
  • Immunoprecipitation of NEK4 from HeLa cell lysate (1mg) using ab95176 at 3µg/mg lysate , followed by Western blotting of 20% of the immunoprecipitate: (1) ab95176 at 1 µg/ml, (2) control IgG
    Detection: Chemiluminescence with exposure times of 30 seconds.

Anti-NEK4 antibody (ab95176)参考文献

ab95176 has not yet been referenced specifically in any publications.

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