概述

  • 产品名称Anti-NEDD4-2抗体
    参阅全部 NEDD4-2 一抗
  • 描述
    兔多克隆抗体to NEDD4-2
  • 经测试应用适用于: IHC-P, ICC, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Xenopus laevis
  • 免疫原

    Recombinant fusion protein (Mouse) LAEDGASGSATNSNNHLVEPQIRRPRSLSSPTVTLSAPLEG AKDSPIRRAVKDTLSNPQSP QPSPYNSPKPQHKVTQ

性能

应用

Our Abpromise guarantee covers the use of ab46521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000.
ICC Use at an assay dependent concentration.
ICC/IF 1/200.
WB 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).

靶标

  • 功能E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.
  • 组织特异性Ubiquitously expressed, with highest levels in prostate, pancreas and kidney.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 4 WW domains.
  • 翻译后修饰Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
    Auto-ubiquitinated.
  • 细胞定位Cytoplasm. May be recruited to exosomes by NDFIP1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin protein ligase NEDD4 like protein antibody
    • E3 ubiquitin-protein ligase NEDD4-like antibody
    • KIAA0439 antibody
    • NED4L_HUMAN antibody
    • NEDD4 2 antibody
    • NEDD4 2c antibody
    • Nedd4-2 antibody
    • NEDD4-2a antibody
    • NEDD4.2 antibody
    • NEDD4b antibody
    • NEDD4L antibody
    • NEDD4La antibody
    • NEDD4Lb antibody
    • NEDD4Lc antibody
    • NEDD4Ld antibody
    • NEDD4Le antibody
    • NEDD4Lf antibody
    • NEDD4Lg antibody
    • NEDD4Lh antibody
    • NEDL3 antibody
    • Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase antibody
    • Neural precursor cell expressed, developmentally down regulated 4 like antibody
    • RSP5 antibody
    • Ubiquitin protein ligase Rsp5 antibody
    see all

Anti-NEDD4-2 antibody 图像

  • All lanes : Anti-NEDD4-2 antibody (ab46521)

    Lane 1 : Extract from non-transfected HEK293 cells
    Lane 2 : Extract from HEK293 cells transfected with NEDD4-2


    Predicted band size : 120 kDa
    Observed band size : 120 kDa
  • ab46251 (1/1000) staining NEDD4-2 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and membrane staining of the intestinal glands cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab46521 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46521, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NEDD4-2 antibody (ab46521)参考文献

This product has been referenced in:
  • Fu L  et al. ?F508 CFTR surface stability is regulated by DAB2 and CHIP-mediated ubiquitination in post-endocytic compartments. PLoS One 10:e0123131 (2015). WB ; Human . Read more (PubMed: 25879443) »
  • Peters DM  et al. TGF-ß directs trafficking of the epithelial sodium channel ENaC which has implications for ion and fluid transport in acute lung injury. Proc Natl Acad Sci U S A 111:E374-83 (2014). Human . Read more (PubMed: 24324142) »

See all 9 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Non-reduced Denaturing (6%)
Sample Human Cell lysate - whole cell (PC3)
Specification PC3
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Wei Cui

Verified customer

提交于 Mar 11 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Kidney)
Specification Kidney
Fixative PLP
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EGTA buffer
Permeabilization Yes - saponin
Blocking step (agent) for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 08 2013

DISCOUNT CODE: ***
Expiration date: March 8th, 2012
Value: ***

I am very pleased to hear you would like to accept our offer and test ab46521 in IHC-P. This code will give you *** off your next order before the expiration date. To red...

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Thank you very much for your interest in ab46521.

To our knowledge, this product has not been tested in PIG. Our BLAST of the immunogen showed 99% homologies. Based on this data we believe that this product should work in pig samples. By p...

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Thank you for your patience.

I have heard back regarding ab46521:
The antibody is specific for Nedd4-2. There is no crossreactivity with Nedd4, also referred to as Nedd4-1.

I hope this information is helpful to you. Please do...

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Thank you for contacting us.

I have heard back regading ab131167: The antibodyimmunogen shares 71% sequence identity with human NEDD4 (Uniprot ID: P46934). We have not confirmed the cross-reactivity of this antibody with NEDD4 by testing. Read More

Vielen Dank für das Senden dieser ausführlicher und kompletter Dokumentation.
Ich habe mir die Bilder und das Protokoll angeschaut, und es scheint mir wirklich komisch, dass der Antikörper sich so verhält. Können Sie bestätigen, dass der sekundäre...

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Vielen Dank für Ihren Anruf.
Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.
Wie am Telefon besprochen, wären wir Ihnen dankbar uns die Protokolldaten mit einem Bild des Resultates zuzusenden. Ich habe Ihnen deshalb u...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (pancreas)
Specification pancreas
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton X100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (pancreas)
Loading amount 40 µg
Specification pancreas
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 11 2011

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"