Anti-NEDD1抗体(ab89738)

概述

  • 产品名称Anti-NEDD1抗体
    参阅全部 NEDD1 一抗
  • 描述
    兔多克隆抗体to NEDD1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 2-51 (

    QENLRFASSGDDIKIWDASSMTLVDKFNPHTSPHGISSICWSSNNNFLVT

    ) of Human NEDD1 (NP_690869)

  • 阳性对照
    • Human fetal spleen lysate

性能

应用

Our Abpromise guarantee covers the use of ab89738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 72 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-NEDD1 antibody 图像

  • Anti-NEDD1 antibody (ab89738) at 1 µg/ml + Human fetal spleen lysate at 10 µg

    Secondary
    HRP anti rabbit IgG at 1/50000 dilution

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 45 kDa,58 kDa. We are unsure as to the identity of these extra bands.

Anti-NEDD1 antibody (ab89738)参考文献

ab89738 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (Spindles in X. laevis cytoplasmic egg extract)
Specification Spindles in X. laevis cytoplasmic egg extract
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Oct 09 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - other (Xenopus laevis cytoplasmic egg extract)
Loading amount 50 µg
Specification Xenopus laevis cytoplasmic egg extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"