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Recombinant fragment: AGVASSLSEK IADSIGNNRQ NAPLTSIQIR FIQNMIQETL DDFREACHRD IVNLQVEMIK QFHMQLNEMH SLLERYSVNE GLVAEIERLR EENKRLRAHF , corresponding to amino acids 561-661 of Human NEDD1
Our Abpromise guarantee covers the use of ab57336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 72 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use 0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22261722|
Overlay histogram showing HeLa cells stained with ab57336 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57336, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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