The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
An additional band was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide and likely represents the mature form.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.