概述

  • 产品名称
    Anti-NEDD1抗体
  • 描述
    山羊多克隆抗体to NEDD1
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    C-NQTRNSEKFEKPEN

    , corresponding to internal sequence amino acids 520-533 of Human NEDD1 (NP_690869.1).

  • 阳性对照
    • Hela cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab56317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
    An additional band was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide and likely represents the mature form.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.
    • 序列相似性
      Contains 8 WD repeats.
    • 细胞定位
      Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ35902 antibody
      • GCP WD antibody
      • NEDD-1 antibody
      • NEDD1 antibody
      • NEDD1_HUMAN antibody
      • Neural precursor cell expressed developmentally down-regulated protein 1 antibody
      • Neural precursor cell expressed, developmentally down regulated gene 1 antibody
      • Protein NEDD1 antibody
      • TUBGCP7 antibody
      see all

    图片

    • Anti-NEDD1 antibody (ab56317) at 0.1 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µg

      Predicted band size : 72 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    实验方案

    文献

    ab56317 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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