Anti-Nectin 3抗体[103-A1] (ab16913)
Key features and details
- Rat monoclonal [103-A1] to Nectin 3
- Suitable for: IHC-Fr, IP, ICC/IF, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Isotype: IgG2a
概述
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产品名称
Anti-Nectin 3抗体[103-A1]
参阅全部 Nectin 3 一抗 -
描述
大鼠单克隆抗体[103-A1] to Nectin 3 -
宿主
Rat -
经测试应用
适用于: IHC-Fr, IP, ICC/IF, Flow Cyt, Functional Studiesmore details
不适用于: WB -
种属反应性
与反应: Mouse
不与反应: Rat, Human -
免疫原
Recombinant fragment, corresponding to Mouse Nectin 3 extracellular domain, amino acids 50-400 fused to IgG Fc.
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阳性对照
- L-cells (A cultured line of C3H mouse fibroblasts)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Provided as a 0.2µm purified antibody solution -
克隆
单克隆 -
克隆编号
103-A1 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16913于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-Fr | (1) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
|
|
ICC/IF | (1) |
Use at an assay dependent concentration.
|
Flow Cyt | (1) |
Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Functional Studies |
Use at an assay dependent concentration.
|
说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Functional Studies
Use at an assay dependent concentration. |
靶标
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功能
Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. -
组织特异性
Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines. -
序列相似性
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 58998 Mouse
- SwissProt: Q9JLB9 Mouse
- Unigene: 328072 Mouse
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别名
- CD113 antibody
- CDw113 antibody
- DKFZP566B0846 antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (5)
ab16913 被引用在 5 文献中.
- Kirjavainen A et al. The Rho GTPase Cdc42 regulates hair cell planar polarity and cellular patterning in the developing cochlea. Biol Open 4:516-26 (2015). PubMed: 25770185
- Lachke SA et al. The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet 131:235-50 (2012). IHC-Fr ; Mouse . PubMed: 21769484
- Lin YW et al. Localization of ubiquitin specific protease 26 at blood-testis barrier and near Sertoli cell-germ cell interface in mouse testes. Int J Androl 34:e368-77 (2011). PubMed: 21449984
- Tanaka-Okamoto M et al. Increased susceptibility to spontaneous lung cancer in mice lacking LIM-domain only 7. Cancer Sci 100:608-16 (2009). PubMed: 19215226
- Toyama Y et al. Disruption of ectoplasmic specializations between Sertoli cells and maturing spermatids by anti-nectin-2 and anti-nectin-3 antibodies. Asian J Androl 10:577-84 (2008). IHC-P ; Mouse . PubMed: 18097512