Anti-Necdin抗体- ChIP Grade (ab18554)

概述

  • 产品名称Anti-Necdin抗体- ChIP Grade
    参阅全部 Necdin 一抗
  • 描述
    兔多克隆抗体to Necdin - ChIP Grade
  • 经测试应用适用于: WB, ChIPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Fusion protein (GST-tag) corresponding to Human Necdin aa 1-143 (N terminal).

  • 阳性对照
    • Jurkat lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab18554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). Non-specific proteins were also detected at 70 and 100kDa, detection of which was inhibited by preincubation with the immunizing peptide.
ChIP Use at an assay dependent dilution. PubMed: 20663865

靶标

  • 功能Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.
  • 组织特异性Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post-mitotic neurons.
  • 序列相似性Contains 1 MAGE domain.
  • 细胞定位Perikaryon. Nucleus. Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HsT16328 antibody
    • NDN antibody
    • NECD_HUMAN antibody
    • Necdin antibody
    • Necdin homolog antibody
    • necdin related protein antibody
    • PWCR antibody
    see all

Anti-Necdin antibody - ChIP Grade 图像



  • Predicted band size : 36 kDa

Anti-Necdin antibody - ChIP Grade (ab18554)参考文献

This product has been referenced in:
  • Wu M  et al. The ciliary protein cystin forms a regulatory complex with necdin to modulate Myc expression. PLoS One 8:e83062 (2013). ChIP . Read more (PubMed: 24349431) »
  • Haviland R  et al. Necdin, a Negative Growth Regulator, Is a Novel STAT3 Target Gene Down-Regulated in Human Cancer. PLoS One 6:e24923 (2011). WB ; Human . Read more (PubMed: 22046235) »

See all 5 Publications for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issuedtwo free of charge replacements:

One vial of ab18554 is being replaced with one vial of ab9574.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"