概述

  • 产品名称Anti-Necdin抗体
    参阅全部 Necdin 一抗
  • 描述
    兔多克隆抗体to Necdin
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide, corresponding to a region within internal sequence amino acids 180-229 (ALSNRMPMTG LLLMILSLIY VKGRGARESA VWNVLRILGL RPWKKHSTFG) of Human Necdin (NP_002478).

  • 阳性对照
    • Fetal brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab94529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.
  • 组织特异性Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post-mitotic neurons.
  • 序列相似性Contains 1 MAGE domain.
  • 细胞定位Perikaryon. Nucleus. Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HsT16328 antibody
    • NDN antibody
    • NECD_HUMAN antibody
    • Necdin antibody
    • Necdin homolog antibody
    • necdin related protein antibody
    • PWCR antibody
    see all

Anti-Necdin antibody 图像

  • Anti-Necdin antibody (ab94529) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa

Anti-Necdin antibody (ab94529)参考文献

ab94529 has not yet been referenced specifically in any publications.

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