概述

  • 产品名称Anti-NECAP2抗体
    参阅全部 NECAP2 一抗
  • 描述
    小鼠多克隆抗体to NECAP2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-263 of Human NECAP2

  • 阳性对照
    • NECAP2 transfected 293T cell lysate; human stomach tissue lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab68747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects bands of approximately 37 and 31 kDa in separate blots(predicted molecular weight: 28 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in endocytosis.
    • 序列相似性Belongs to the NECAP family.
    • 结构域The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself.
    • 细胞定位Cytoplasmic vesicle, clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Adaptin ear binding coat associated protein 2 antibody
      • Adaptin ear-binding coat-associated protein 2 antibody
      • FLJ10420 antibody
      • NECAP endocytosis associated 2 antibody
      • NECAP endocytosis associated protein 2 antibody
      • NECAP endocytosis-associated protein 2 antibody
      • NECAP-2 antibody
      • NECAP2 antibody
      • NECP2_HUMAN antibody
      see all

    Anti-NECAP2 antibody 图像

    • All lanes : Anti-NECAP2 antibody (ab68747) at 1/500 dilution

      Lane 1 : NECAP2 transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/5000 dilution

      Predicted band size : 28 kDa
      Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    • Anti-NECAP2 antibody (ab68747) at 1/500 dilution + NECAP2 expressed in human stomach at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/5000 dilution

      Predicted band size : 28 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    Anti-NECAP2 antibody (ab68747)参考文献

    ab68747 has not yet been referenced specifically in any publications.

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