概述

  • 产品名称
    Anti-NECAP1抗体
  • 描述
    兔多克隆抗体to NECAP1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 90-139 (RYFVIRIQDG TGRSAFIGIG FTDRGDAFDF NVSLQDHFKW VKQETEISKE) of Mouse NECAP1 protein (NP_080543).

  • 阳性对照
    • Mouse spleen lysate

性能

应用

Our Abpromise guarantee covers the use of ab112090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Involved in endocytosis.
  • 序列相似性
    Belongs to the NECAP family.
  • 结构域
    The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself. The WXXF motif 1, which is preceded by an acidic residue and has a glycine in second position mediates specific interaction with AP-1. The WXXF motif 2, which is followed by the C-terminal carboxyl group negative charge, allows specific interaction with AP-2.
  • 细胞定位
    Cytoplasmic vesicle > clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adaptin ear binding coat associated protein 1 antibody
    • Adaptin ear-binding coat-associated protein 1 antibody
    • DKFZP566B183 antibody
    • MGC131900 antibody
    • NECAP 1 antibody
    • NECAP endocytosis associated 1 antibody
    • NECAP endocytosis-associated protein 1 antibody
    • NECAP-1 antibody
    • NECAP1 antibody
    • NECP1_HUMAN antibody
    see all

图片

  • Anti-NECAP1 antibody (ab112090) at 1 µg/ml + Mouse spleen lysate at 10 µg

    Predicted band size : 30 kDa

实验方案

文献

ab112090 has not yet been referenced specifically in any publications.

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