概述

  • 产品名称Anti-NECAP1抗体
    参阅全部 NECAP1 一抗
  • 描述
    兔多克隆抗体to NECAP1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 90-139 (RYFVIRIQDG TGRSAFIGIG FTDRGDAFDF NVSLQDHFKW VKQETEISKE) of Mouse NECAP1 protein (NP_080543).

  • 阳性对照
    • Mouse spleen lysate

性能

应用

Our Abpromise guarantee covers the use of ab112090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Involved in endocytosis.
  • 序列相似性Belongs to the NECAP family.
  • 结构域The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself. The WXXF motif 1, which is preceded by an acidic residue and has a glycine in second position mediates specific interaction with AP-1. The WXXF motif 2, which is followed by the C-terminal carboxyl group negative charge, allows specific interaction with AP-2.
  • 细胞定位Cytoplasmic vesicle > clathrin-coated vesicle membrane. Cell membrane. Colocalizes with AP-2 at the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adaptin ear binding coat associated protein 1 antibody
    • Adaptin ear-binding coat-associated protein 1 antibody
    • DKFZP566B183 antibody
    • MGC131900 antibody
    • NECAP 1 antibody
    • NECAP endocytosis associated 1 antibody
    • NECAP endocytosis-associated protein 1 antibody
    • NECAP-1 antibody
    • NECAP1 antibody
    • NECP1_HUMAN antibody
    see all

Anti-NECAP1 antibody 图像

  • Anti-NECAP1 antibody (ab112090) at 1 µg/ml + Mouse spleen lysate at 10 µg

    Predicted band size : 30 kDa

Anti-NECAP1 antibody (ab112090)参考文献

ab112090 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"