Anti-Nebulin抗体[NB2] (ab11099)

概述

  • 产品名称Anti-Nebulin抗体[NB2]
    参阅全部 Nebulin 一抗
  • 描述
    小鼠单克隆抗体[NB2] to Nebulin
  • 特异性Does not cross react with titin. Monoclonal Anti-Nebulin reacts only with skeletal muscle, not with smooth muscle or different non-muscle tissues and cultured cells. The following is a summary of the antibody reactivity on various muscles in immunofluorescence using frozen section (a), isolated myofibrils (b), or immunoblotting (c): Fish skeletal muscle (a) (Torpedo spec.) + Toad skeletal muscle (a) (Bufo marinus) + Salamander skeletal muscle (c) (Ambystoma tigrina) + Salamander heart muscle (a) - Alligator skeletal muscle (a)(Alligator mississippiensis) + Pigeon skeletal muscle (b) (c) + Chicken skeletal muscle (b) (c) + Chicken heart muscle (c) - Chicken gizzard smooth muscle (c) - Mouse skeletal muscle (a) + Rat skeletal muscle (b) (c) + Rat heart muscle (a) - Rat rhabdomyosarcoma (a) + Rabbit skeletal muscle (b) + Bovine heart muscle (a) - Pig skeletal muscle (c) + Human skeletal muscle (a) +
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    Reacts with a wide range of species, including chicken, mouse rat, rabbit, cow, pig, human, salamander, pigeon, alligator, toad and fish. Not yet tested in other species.
  • 免疫原

    Full length native protein (purified) (Chicken).

  • 常规说明If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab11099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明IF: 1/400.
    WB: Use at an assay dependent dilution. Detects a band of approximately 500 kDa.

    By indirect immunofluorescence, the antibody shows a typical striated staining pattern of frozen sections of chicken skeletal muscle. It stains within the I-band and shows a decoration line at a distance of 0.22 µm from the z-line in electron micrographs.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Bind and stabilize F-actin.
    • 组织特异性Muscle specific. Located in the thin filament of striated muscle.
    • 疾病相关Defects in NEB are the cause of nemaline myopathy type 2 (NEM2) [MIM:256030]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination.
    • 序列相似性Contains 178 nebulin repeats.
      Contains 1 SH3 domain.
    • 细胞定位Cytoplasm > myofibril > sarcomere. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 别名
      • DKFZp686C1456 antibody
      • FLJ11505 antibody
      • FLJ36536 antibody
      • FLJ39568 antibody
      • FLJ39584 antibody
      • NEB 177D antibody
      • NEB antibody
      • NEB177D antibody
      • NEBU_HUMAN antibody
      • Nebulin antibody
      • NEM 2 antibody
      • NEM2 antibody
      see all

    Anti-Nebulin antibody [NB2] (ab11099)参考文献

    ab11099 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Cat Cell lysate - other (Fecal protein)
    Gel Running Conditions Reduced Non-Denaturing (Native)
    Loading amount 18 µg
    Specification Fecal protein
    Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 16 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"