Anti-Ndufs4抗体[2C7CD4AG3] (ab87399)
Key features and details
- Mouse monoclonal [2C7CD4AG3] to Ndufs4
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
概述
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产品名称
Anti-Ndufs4抗体[2C7CD4AG3]
参阅全部 Ndufs4 一抗 -
描述
小鼠单克隆抗体[2C7CD4AG3] to Ndufs4 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, WBmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Recombinant full length protein. This information is considered to be commercially sensitive.
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阳性对照
- WB: isolated mitochondria from human, bovine, rat, and mouse heart tissue. Flow Cyt: HepG2 cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES, Sodium chloride -
Concentration information loading...
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纯度
IgG fraction -
纯化说明
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
2C7CD4AG3 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab87399于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt | (1) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
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说明 |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
靶标
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功能
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
疾病相关
Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
序列相似性
Belongs to the complex I NDUFS4 subunit family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 327680 Cow
- Entrez Gene: 4724 Human
- Entrez Gene: 17993 Mouse
- Entrez Gene: 499529 Rat
- Omim: 602694 Human
- SwissProt: Q02375 Cow
- SwissProt: O43181 Human
- SwissProt: Q9CXZ1 Mouse
see all -
别名
- AQDQ antibody
- CI 18 antibody
- CI 18 kDa antibody
see all
图片
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All lanes : Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)
Lane 1 : Isolated mitochondria from human heart at 15 µg
Lane 2 : Isolated mitochondria from bovine heart at 6 µg
Lane 3 : Isolated mitochondria from rat heart at 30 µg
Lane 4 : Isolated mitochondria from mouse heart at 30 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Overlay histogram showing HepG2 cells stained with ab87399 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87399, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab87399 被引用在 18 文献中.
- Liu Y et al. Gene therapy restores mitochondrial function and protects retinal ganglion cells in optic neuropathy induced by a mito-targeted mutant ND1 gene. Gene Ther 29:368-378 (2022). PubMed: 35383288
- Pagano Zottola AC et al. Expression of Functional Cannabinoid Type-1 (CB1) Receptor in Mitochondria of White Adipocytes. Cells 11:N/A (2022). PubMed: 36010658
- Bertan F et al. Comparative analysis of CI- and CIV-containing respiratory supercomplexes at single-cell resolution. Cell Rep Methods 1:100002 (2021). PubMed: 35474694
- Esparza-Moltó PB et al. Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition. PLoS Biol 19:e3001252 (2021). PubMed: 33983919
- Hernansanz-Agustín P et al. Na+ controls hypoxic signalling by the mitochondrial respiratory chain. Nature 586:287-291 (2020). PubMed: 32728214