Anti-Ndufs4抗体[2C7CD4AG3] (ab87399)

概述

  • 产品名称
    Anti-Ndufs4抗体[2C7CD4AG3]
    参阅全部 Ndufs4 一抗
  • 描述
    小鼠单克隆抗体[2C7CD4AG3] to Ndufs4
  • 经测试应用
    适用于: Flow Cyt, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Recombinant full length protein (Human)

  • 常规说明

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab87399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

靶标

  • 功能
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • 疾病相关
    Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • 序列相似性
    Belongs to the complex I NDUFS4 subunit family.
  • 细胞定位
    Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AQDQ antibody
    • CI 18 antibody
    • CI 18 kDa antibody
    • CI AQDQ antibody
    • CI-18 kDa antibody
    • CI-AQDQ antibody
    • Complex I 18 kDa antibody
    • Complex I AQDQ antibody
    • Complex I-18 kDa antibody
    • Complex I-AQDQ antibody
    • mitochondrial antibody
    • mitochondrial respiratory chain complex I (18 KD subunit) antibody
    • NADH coenzyme Q reductase antibody
    • NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa antibody
    • NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 antibody
    • NADH dehydrogenase antibody
    • NADH ubiquinone oxidoreductase 18 kDa subunit antibody
    • NADH-ubiquinone oxidoreductase 18 kDa subunit antibody
    • NDUFS4 antibody
    • NDUS4_HUMAN antibody
    see all

Anti-Ndufs4 antibody [2C7CD4AG3] 图像

  • All lanes : Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)

    Lane 1 : Isolated mitochondria from human heart at 15 µg
    Lane 2 : Isolated mitochondria from bovine heart at 6 µg
    Lane 3 : Isolated mitochondria from rat heart at 30 µg
    Lane 4 : Isolated mitochondria from mouse heart at 30 µg


    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • Overlay histogram showing HepG2 cells stained with ab87399 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87399, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)参考文献

This product has been referenced in:
  • Hernansanz-Agustín P  et al. Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia. Redox Biol 12:1040-1051 (2017). WB . Read more (PubMed: 28511347) »
  • Wang M  et al. Mitochondrial complex I deficiency leads to the retardation of early embryonic development in Ndufs4 knockout mice. PeerJ 5:e3339 (2017). Read more (PubMed: 28533980) »

See all 10 Publications for this product

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Dec 24 2014

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (MDM)
Specification
MDM
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Nov 14 2013

Application
Flow Cytometry
Fixation
Paraformaldehyde
Sample
Human Cell (MDM)
Specification
MDM
Gating Strategy
LIVE CELL
Permeabilization
Yes - PBS TWEEN
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Abcam user community

Verified customer

提交于 Nov 13 2013

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Mouse Cell lysate - whole cell (bmdm)
Specification
bmdm
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Abcam user community

Verified customer

提交于 Nov 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - other (Mitochondrial fraction)
Loading amount
50 µg
Specification
Mitochondrial fraction
Gel Running Conditions
Reduced Denaturing (14%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 30 2013

Thank you very much for your interest in ab87399.

To our knowledge, ab87399 has not been tested in IHC.Therefore, I can offer a discount off a future purchase if you buy ab87399 now, test it inIHC and submit feedback to us in the form of an ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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