Anti-NDUFS3抗体[3F9DD2] (ab110246)

概述

  • 产品名称
    Anti-NDUFS3抗体[3F9DD2]
    参阅全部 NDUFS3 一抗
  • 描述
    小鼠单克隆抗体[3F9DD2] to NDUFS3
  • 经测试应用
    适用于: IHC-Fr, IHC-Glut, Flow Cyt, ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Zebrafish
  • 免疫原

    Whole mitochondria from Human Heart.

  • 阳性对照
    • Isolated mitochondria from Human, Bovine Rat and Mouse hearts; Cultured Human fibroblasts; HeLa cells; Human colon and cerebellum tissues.
  • 常规说明

    Alternative versions available:

    Anti-NDUFS3 antibody (Alexa Fluor® 647) [3F9DD2] (ab197970)

    Anti-NDUFS3 antibody (HRP) [3F9DD2] (ab197971)

     

    Product was previously marketed under the MitoSciences sub-brand.

     

性能

应用

Our Abpromise guarantee covers the use of ab110246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 25223649
IHC-Glut 1/100.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 5 - 10 µg/ml.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 30 kDa.

靶标

  • 功能
    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • 序列相似性
    Belongs to the complex I 30 kDa subunit family.
  • 细胞定位
    Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CI 30 antibody
    • CI 30KD antibody
    • CI-30kD antibody
    • Complex I 30KD antibody
    • Complex I 30kDa subunit antibody
    • COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT antibody
    • Complex I-30kD antibody
    • mitochondrial antibody
    • NADH coenzyme Q reductase antibody
    • NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa antibody
    • NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial antibody
    • NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 antibody
    • NADH dehydrogenase ubiquinone 30 kDa subunit antibody
    • NADH-ubiquinone oxidoreductase 30 kDa subunit antibody
    • NADH-Ubiquinone Oxidoreductase Fe-S Protein 3 antibody
    • NDUFS3 antibody
    • NDUS3_HUMAN antibody
    see all

Anti-NDUFS3 antibody [3F9DD2] 图像

  • All lanes : Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 1/1000 dilution

    Lane 1 : Whole cell lysates from NDUFS3 KO cells.
    Lane 2 : Whole cell lysates from NDUFS3 KO cells.
    Lane 3 : Whole cell lysates from NDUFS3 WT cells.
    Lane 4 : Whole cell lysates from NDUFS3 WT cells.
    Lane 5 : Isolated mitochondria from NDUFS3 KO cells.
    Lane 6 : Isolated mitochondria from NDUFS3 KO cells.
    Lane 7 : Isolated mitochondria from NDUFS3 WT cells.
    Lane 8 : Isolated mitochondria from NDUFS3 WT cells.

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat anti-Mouse monoclonal HRP conjugate at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Additional bands at : 260 kDa (possible non-specific binding),40 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous AbReview

    See Abreview

  • All lanes : Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 0.5 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg


    Predicted band size : 30 kDa
  • ab110246, at 10 µg/ml, staining NDUFS3 in cultured Human fibroblasts by Immunofluorescence. The cells were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110246, followed by a fluorescent goat-anti-mouse IgG.
  • ab110246, at 1 µg/ml, staining NDUFS3 in HeLa cells by Flow Cytometry (Blue).

    Isotype control antibody, at 1 µg/ml, staining (red).

  • ab110246, at 1/100, staining NDUFS3 in Human colon tissue fixed with 4% PFA by Immunohistochemistry.

    ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated for 1 hour with the target. Antigen retrieval was not required.

  • ab110246, at 1/1000, staining NDUFS3 in formalin fixed and paraffin embedded Human cerebellum tissue by Immunohistochemistry.

    ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated with the target for 1 hour. Antigen retrieval performed by 1 min pressure cooking with 1mmol EDTA, pH8.

  • ab110246 staining NDUFS3 in Human colon tissue sections from a normal ageing patient by Immunohistochemistry. Kindly provided by Dr. L. Greaves and D. Turnbull, Mitochondrial Research Group, Newcastle University. For more details, see Taylor et al., J. Clin. Invest. 112:1351-1360 (2003).

Anti-NDUFS3 antibody [3F9DD2] (ab110246)参考文献

This product has been referenced in:

See all 33 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - other (osteosarcoma)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
osteosarcoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Nov 03 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Chinese Hamster Cell lysate - whole cell (CHO cell)
Specification
CHO cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Feb 10 2015

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Total protein in input
30 µg
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提交于 Jan 07 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Permeabilization
Yes - 0.2% Triton-X100
Fixative
Paraformaldehyde
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提交于 Nov 24 2014

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient Tris-Gly gel)
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Blocking step
LI-COR­ Odyssey­ Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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提交于 Sep 19 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient Tris-Gly gel)
Sample
Mouse Cell lysate - whole cell (MEFs)
Specification
MEFs
Blocking step
LI-COR­ Odyssey­ Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

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Verified customer

提交于 Sep 19 2014

Thank you for your reply.
For a use on tissue sections I can recommend to use an antibody directed against NDUFS3 (or Complex I 30kDa subunit) such as ab110246. The link to the datasheet of this antibody is www.abcam.com/ab110246.
I hope this...

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Thank you very much for contacting us with your question.
The lab does use an antigen retrieval step while staining cultured cells with ab110246, so I would recommend using this step. Our ICC protocol with antigen retrieval can be found at this li...

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Thank you for contacting us.

That’s right we immunized the whole complex. This protein is very immunoreactive, we have generated many monoclonals against this subunit.

Subunits are large compared to the epitope of an antibody bi...

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Thank you for contacting us.

We prepared this antibody by immunization of the entire complex NADH dehydrogenase.The reactivity with NDUFS3 was proved by reaction with recombinant human NDUFS3 expressed in E.coli.Unfortunately we don’t know t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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