重组Anti-Ndufs1抗体[EPR11522(B)] (ab157221)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11522(B)] to Ndufs1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Ndufs1抗体[EPR11522(B)]
参阅全部 Ndufs1 一抗 -
描述
兔单克隆抗体[EPR11522(B)] to Ndufs1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Raji, HepG2, MOLT4 and Jurkat cell lysates; Human kidney and liver tissues; HepG2 cells; Permeabilized Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11522(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab157221于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 79 kDa.
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ICC/IF |
1/50 - 1/100.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 79 kDa. |
ICC/IF
1/50 - 1/100. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. -
疾病相关
Defects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
序列相似性
Belongs to the complex I 75 kDa subunit family.
Contains 1 2Fe-2S ferredoxin-type domain. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4719 Human
- Entrez Gene: 227197 Mouse
- Entrez Gene: 301458 Rat
- Omim: 157655 Human
- SwissProt: P28331 Human
- SwissProt: Q91VD9 Mouse
- SwissProt: Q66HF1 Rat
- Unigene: 471207 Human
see all -
别名
- CI-75kD antibody
- Complex I 75Kd antibody
- Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
see all
图片
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All lanes : Anti-Ndufs1 antibody [EPR11522(B)] (ab157221) at 1/10000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 79 kDa -
Immunofluorescent analysis of HepG2 cells labeling Ndufs1 with ab157221 at 1/50 dilution.
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ab157221 showing +ve staining in Human normal prostate.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab157221 showing +ve staining in Human heart.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab157221 showing +ve staining in Human cervical carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab157221 showing +ve staining in Human normal brain.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ndufs1 with ab157221 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ndufs1 with ab157221 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling Ndufs1 with ab157221 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab157221 被引用在 6 文献中.
- Wagner C et al. KIAA1363 affects retinyl ester turnover in cultured murine and human hepatic stellate cells. J Lipid Res 63:100173 (2022). PubMed: 35101424
- Pinson MR et al. Gag-like proteins: Novel mediators of prenatal alcohol exposure in neural development. Alcohol Clin Exp Res 46:556-569 (2022). PubMed: 35187673
- Colaço HG et al. Tetracycline Antibiotics Induce Host-Dependent Disease Tolerance to Infection. Immunity 54:53-67.e7 (2021). PubMed: 33058782
- Li Y et al. CISD3 inhibition drives cystine-deprivation induced ferroptosis. Cell Death Dis 12:839 (2021). PubMed: 34497268
- Schweiger M et al. Pharmacological inhibition of adipose triglyceride lipase corrects high-fat diet-induced insulin resistance and hepatosteatosis in mice. Nat Commun 8:14859 (2017). WB . PubMed: 28327588
- Petruzzelli M et al. A switch from white to brown fat increases energy expenditure in cancer-associated cachexia. Cell Metab 20:433-47 (2014). PubMed: 25043816