Anti-NDUFB8抗体[20E9DH10C12] (ab110242)

概述

  • 产品名称Anti-NDUFB8抗体[20E9DH10C12]
    参阅全部 NDUFB8 一抗
  • 描述
    小鼠单克隆抗体[20E9DH10C12] to NDUFB8
  • 经测试应用适用于: IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    NDUFB8 from Cow

  • 阳性对照
    • Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart. Skeletal muscle tissue.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 22 kDa.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • 序列相似性Belongs to the complex I NDUFB8 subunit family.
  • 细胞定位Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ASHI antibody
    • CI-ASHI antibody
    • Complex I ASHI subunit antibody
    • Complex I-ASHI antibody
    • mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody
    • NADH-ubiquinone oxidoreductase ASHI subunit antibody
    • NDUB8_HUMAN antibody
    • NDUFB8 antibody
    see all

Anti-NDUFB8 antibody [20E9DH10C12] 图像

  • Skeletal muscle immunohistochemistry using ab110242 on frozen tissue sections from a patient with a single large deletion of the mtDNA show a mosaic of complex I positive and complex I negative fibers.
  • All lanes : Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) at 0.5 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from cow heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg
    Lane 4 : Isolated mitochondria from mouse heart at 10 µg


    Predicted band size : 22 kDa
    Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)参考文献

This product has been referenced in:
  • Pérez-Pérez R  et al. COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation. Cell Rep 16:2387-98 (2016). WB . Read more (PubMed: 27545886) »
  • Kauppila JH  et al. A Phenotype-Driven Approach to Generate Mouse Models with Pathogenic mtDNA Mutations Causing Mitochondrial Disease. Cell Rep 16:2980-90 (2016). Read more (PubMed: 27626666) »

See all 45 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Human brain lysate)
Gel Running Conditions Reduced Denaturing (4-12)
Loading amount 10 µg
Specification Human brain lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Sandrine Wauters

Verified customer

提交于 Apr 01 2016

Application Western blot
Sample Mouse Cell lysate - other (Mouse mitos from liver (A) and muscle (B))
Gel Running Conditions Non-reduced Non-Denaturing (Native) (3-12%)
Loading amount 10 µg
Specification Mouse mitos from liver (A) and muscle (B)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 29 2015

Thank you for contacting us.

The MSDS for these products have been added to each product datasheet. They should appear on the website within the next few hours (time for the cache to be regenerated).

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain- Cerebellum)
Specification Brain- Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH8.0 EDTA
Permeabilization No
Username

Ms. Philippa Hepplewhite

Verified customer

提交于 Jun 28 2012

Thank you for your call earlier.

I now had a look at the homology between the immunogens of the antibodies included in the MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail (ab110413) and the respective proteins in Drosophila.
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Thank you for this additional information. I can confirm that ab110413 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail) contains 5 antibodies. The datasheets and more information about these antibodies can be found when following...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"