概述

  • 产品名称Anti-NDUFB10抗体
    参阅全部 NDUFB10 一抗
  • 描述
    小鼠多克隆抗体to NDUFB10
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full-length human protein, NDUFB10 (NP_004539.1, 172 a.a).

  • 阳性对照
    • NDUFB10 transfected 293T cell lysate and human kidney lysate

应用

Our Abpromise guarantee covers the use of ab70206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects bands of approximately 19 and 23 kDa (predicted molecular weight: 21 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
    • 序列相似性Belongs to the complex I NDUFB10 subunit family.
    • 细胞定位Mitochondrion inner membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 0610011B04Rik antibody
      • 22kDa antibody
      • CI PDSW antibody
      • CI-PDSW antibody
      • Complex I PDSW antibody
      • Complex I PDSW subunit antibody
      • Complex I-PDSW antibody
      • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 10 22kDa antibody
      • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 antibody
      • NADH ubiquinone oxidoreductase PDSW subunit antibody
      • NADH-ubiquinone oxidoreductase PDSW subunit antibody
      • NDUBA_HUMAN antibody
      • NDUFB 10 antibody
      • Ndufb10 antibody
      • PDSW antibody
      see all

    Anti-NDUFB10 antibody 图像

    • Anti-NDUFB10 antibody (ab70206) at 1/500 dilution + human kidneys at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 21 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-NDUFB10 antibody (ab70206) at 1/500 dilution

      Lane 1 : transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 21 kDa
      Observed band size : 19 kDa (why is the actual band size different from the predicted?)

    Anti-NDUFB10 antibody (ab70206)参考文献

    This product has been referenced in:
    • Hebert-Chatelain E  et al. Preservation of NADH ubiquinone-oxidoreductase activity by Src kinase-mediated phosphorylation of NDUFB10. Biochim Biophys Acta 1817:718-25 (2012). Read more (PubMed: 22321370) »

    See 1 Publication for this product

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