概述

  • 产品名称Anti-NDUFAF1抗体[EPR2795]
    参阅全部 NDUFAF1 一抗
  • 描述
    兔单克隆抗体[EPR2795] to NDUFAF1
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human NDUFAF1

  • 阳性对照
    • HeLa, 293T, A549 and HepG2 cell lysates. Human liver tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab92454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 38 kDa.
IP 1/50.
IHC-P 1/100 - 1/250.
Flow Cyt 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/1000.

靶标

  • 功能Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the CIA30 family.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CGI 65 antibody
    • CGI65 antibody
    • CIA30 antibody
    • CIA30_HUMAN antibody
    • Complex I intermediate associated protein 30 antibody
    • Complex I intermediate associated protein 30 mitochondrial antibody
    • Complex I intermediate-associated protein 30, mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 1 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 antibody
    • NADH:ubiquinone oxidoreductase complex assembly factor 1 antibody
    • NDUFAF 1 antibody
    • Ndufaf1 antibody
    see all

Anti-NDUFAF1 antibody [EPR2795] 图像

  • All lanes : Anti-NDUFAF1 antibody [EPR2795] (ab92454) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 38 kDa
  • Paraffin embedded Human liver tissue labelled with ab92454 at 1/100 dilution.
  • Immunofluorescence analysis of HeLa cells labelled with ab92454 at 1/500 dilution.

Anti-NDUFAF1 antibody [EPR2795] (ab92454)参考文献

This product has been referenced in:
  • Lindfors C  et al. Hypothalamic mitochondrial dysfunction associated with anorexia in the anx/anx mouse. Proc Natl Acad Sci U S A 108:18108-13 (2011). WB . Read more (PubMed: 22025706) »

See 1 Publication for this product

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