概述

  • 产品名称Anti-NDUFA8抗体
    参阅全部 NDUFA8 一抗
  • 描述
    兔多克隆抗体to NDUFA8
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence of human NDUFA8.

  • 阳性对照
    • Human pancreas tissue. Extracts from MCF-7 cells, 293 cells, HUVEC cells, HepG2 cells and Jurkat cells.

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应用

Our Abpromise guarantee covers the use of ab74126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • 序列相似性Belongs to the complex I NDUFA8 subunit family.
    Contains 2 CHCH domains.
  • 结构域Contains four C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.
  • 翻译后修饰May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions.
  • 细胞定位Mitochondrion. Mitochondrion intermembrane space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CI 19KD antibody
    • CI PGIV antibody
    • CI-19kD antibody
    • CI-PGIV antibody
    • Complex I 19k antibody
    • Complex I 19kD antibody
    • Complex I PGIV antibody
    • Complex I PGIV subunit antibody
    • Complex I-19kD antibody
    • Complex I-PGIV antibody
    • MGC793 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 (19kD PGIV) antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 19kDa antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 antibody
    • NADH ubiquinone oxidoreductase 19 kDa subunit antibody
    • NADH ubiquinone oxidoreductase PGIV subunit antibody
    • NADH-ubiquinone oxidoreductase 19 kDa subunit antibody
    • NDUA8_HUMAN antibody
    • NDUFA 8 antibody
    • NDUFA8 antibody
    • OTTHUMP00000022034 antibody
    • PGIV antibody
    see all

Anti-NDUFA8 antibody 图像

  • Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using ab74126, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • All lanes : Anti-NDUFA8 antibody (ab74126) at 1/500 dilution

    Lane 1 : Extracts from MCF-7 cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from HUVEC cells
    Lane 4 : Extracts from HepG2 cells
    Lane 5 : Extracts from Jurkat cells
    Lane 6 : Extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 20 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)

Anti-NDUFA8 antibody (ab74126)参考文献

ab74126 has not yet been referenced specifically in any publications.

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