The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 37-45 kDa (predicted molecular weight: 41 kDa).
Use a concentration of 1 µg/ml.
May be involved in dendritic cell and neuron differentiation. May have anti-tumor activity.
Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level).
Belongs to the NDRG family.
Specifically expressed during dendritic cell differentiation (in vitro). Expression is low in fetal brain and increases during brain postnatal development.
Cytoplasm. Cytoplasm > perinuclear region. Perinuclear in neurons.
All lanes : Anti-NDRG2 antibody (ab60097) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Human heart tissue lysate - total protein (ab29431) Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 4 : Brain (Mouse) Tissue Lysate Lane 5 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
There are five known isoforms of NDRG2 protein. Isoforms 1-5 are 41, 39, 40, 36 and 37 kDa respectively. Ab60097 is predicted to react with all 5 isoforms. This might explain the banding pattern observed.
ICC/IF image of ab60097 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab60097 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.