概述

  • 产品名称Anti-NDRG1抗体
    参阅全部 NDRG1 一抗
  • 描述
    山羊多克隆抗体to NDRG1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    GNSAGPKSMEVSC

    , corresponding to C terminal amino acids 382-394 of Human NDRG1

性能

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应用

Our Abpromise guarantee covers the use of ab37897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 43 kDa.
ELISA 1/3200. Peptide ELISA
IHC-P Use at an assay dependent concentration.

靶标

  • 功能May have a growth inhibitory role.
  • 组织特异性Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
  • 疾病相关Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
  • 序列相似性Belongs to the NDRG family.
  • 细胞定位Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 42 kDa antibody
    • Anti GC4 antibody
    • cap43 antibody
    • cmt4d antibody
    • Differentiation related gene1 protein antibody
    • Differentiation-related gene 1 protein antibody
    • Drg 1 antibody
    • DRG-1 antibody
    • drg1 antibody
    • gc4 antibody
    • GC4 antibody antibody
    • hmsnl antibody
    • Human mRNA for RTP complete cds antibody
    • N myc downstream regulated gene 1 antibody
    • N myc downstream regulated gene 1 protein antibody
    • N-myc downstream-regulated gene 1 protein antibody
    • Ndr 1 antibody
    • ndr1 antibody
    • NDRG 1 antibody
    • Ndrg1 antibody
    • NDRG1 protein antibody
    • NDRG1_HUMAN antibody
    • Nickel specific induction protein antibody
    • Nickel specific induction protein Cap43 antibody
    • Nickel-specific induction protein Cap43 antibody
    • nmsl antibody
    • Nmyc downstream regulated antibody
    • Nmyc downstream regulated gene1 antibody
    • Nmyc downstream regulated gene1 protein antibody
    • Protein NDRG1 antibody
    • Protein regulated by oxygen 1 antibody
    • Protein regulated by oxygen1 antibody
    • Proxy1 antibody
    • Reduced in tumor antibody
    • Reducin antibody
    • Reducing agents and tunicamycin responsive protein antibody
    • Reducing agents and tunicamycin-responsive protein antibody
    • Rit42 antibody
    • RTP antibody
    • targ1 antibody
    • TDD5 antibody
    • tdds antibody
    • Tunicamycin responsive protein antibody
    see all

Anti-NDRG1 antibody 图像

  • Anti-NDRG1 antibody (ab37897) at 0.1 µg/ml + Human Kidney lysate (RIPA buffer, 35µg total protein per lane).

    Predicted band size : 43 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Primary incubated for 1 hour. Detected by western blot using chemiluminescence. A minor band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide.

Anti-NDRG1 antibody (ab37897)参考文献

This product has been referenced in:
  • Drelon C  et al. PKA inhibits WNT signalling in adrenal cortex zonation and prevents malignant tumour development. Nat Commun 7:12751 (2016). IHC-P ; Mouse . Read more (PubMed: 27624192) »
  • Lee JE & Kim JH Valproic acid inhibits the invasion of PC3 prostate cancer cells by upregulating the metastasis suppressor protein NDRG1. Genet Mol Biol 38:527-33 (2015). WB ; Human . Read more (PubMed: 26692161) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (liver)
Loading amount 30 µg
Specification liver
Treatment hypoxia treatment for 24h
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"