概述

  • 产品名称Anti-NDP抗体
    参阅全部 NDP 一抗
  • 描述
    兔多克隆抗体to NDP
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A synthetic peptide corresponding to a region within internal sequence amino acids 36 - 85 (PRRCMRHHYV DSISHPLYKC SSKMVLLARC EGHCSQASRS EPLVSFSTVL) of Human NDP (NP_000257).

  • 阳性对照
    • Human fetal liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab90690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 15 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Activates the canonical Wnt signaling pathway through FZD4 and LRP5 coreceptor. Plays a central role in retinal vascularization by acting as a ligand for FZD4 that signals via stabilizing beta-catenin (CTNNB1) and activating LEF/TCF-mediated transcriptional programs. Acts in concert with TSPAN12 to activate FZD4 independently of the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1). May be involved in a pathway that regulates neural cell differentiation and proliferation. Possible role in neuroectodermal cell-cell interaction.
  • 组织特异性Expressed in the outer nuclear, inner nuclear and ganglion cell layers of the retina, and in fetal and adult brain.
  • 疾病相关Defects in NDP are the cause of Norrie disease (ND) [MIM:310600]; also known as atrophia bulborum hereditaria or Episkopi blindness. ND is a recessive disorder characterized by very early childhood blindness due to degenerative and proliferative changes of the neuroretina. Approximately 50% of patients show some form of progressive mental disorder, often with psychotic features, and about one-third of patients develop sensorineural deafness in the second decade. In addition, some patients have more complex phenotypes, including growth failure and seizure.
    Defects in NDP are the cause of vitreoretinopathy exudative type 2 (EVR2) [MIM:305390]. EVR2 is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. Clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease related abnormality is an arc of avascular retina in the extreme temporal periphery.
  • 序列相似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EVR2 antibody
    • Exudative vitreoretinopathy 2 (X linked) antibody
    • FEVR antibody
    • ND antibody
    • NDP antibody
    • NDP_HUMAN antibody
    • Norrie disease (pseudoglioma) antibody
    • Norrie disease protein antibody
    • Norrin antibody
    • Norrin precursor antibody
    • X linked exudative vitreoretinopathy 2 protein antibody
    • X-linked exudative vitreoretinopathy 2 protein antibody
    see all

Anti-NDP antibody 图像

  • Anti-NDP antibody (ab90690) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 15 kDa
    Gel concentration: 10-20%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cortex tissue labelling NDP with ab90690 at 5µg/ml.

Anti-NDP antibody (ab90690)参考文献

ab90690 has not yet been referenced specifically in any publications.

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