概述

  • 产品名称Anti-NDE1抗体
    参阅全部 NDE1 一抗
  • 描述
    兔多克隆抗体to NDE1
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A synthetic peptide corresponding to a region of Human NDE1

  • 阳性对照
    • HepG2 cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab76384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Required for centrosome duplication. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex (By similarity). Required for formation and function of the mitotic spindle.
  • 序列相似性Belongs to the nudE family.
  • 翻译后修饰Phosphorylated in mitosis.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Localizes to the interphase centrosome and to the mitotic spindle. Concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase in a CENPF-dependent manner. Also localizes to the cleavage furrow during cytokinesis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ20101 antibody
    • HOM TES 87 antibody
    • LIS1 interacting protein NUDE1 rat homolog antibody
    • LIS1 interacting protein NUDE1 antibody
    • LIS4 antibody
    • NDE 1 antibody
    • NDE1 antibody
    • NDE1_HUMAN antibody
    • Nuclear distribution gene E homolog 1 antibody
    • Nuclear distribution protein nudE homolog 1 antibody
    • NUDE 1 antibody
    • NudE antibody
    • NudE nuclear distribution gene E homolog 1 (A. nidulans) antibody
    • NudE nuclear distribution gene E homolog 1 antibody
    • NUDE1 antibody
    see all

Anti-NDE1 antibody 图像

  • Anti-NDE1 antibody (ab76384) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

Anti-NDE1 antibody (ab76384)参考文献

ab76384 has not yet been referenced specifically in any publications.

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