概述

  • 产品名称Anti-NDC1抗体
  • 描述
    兔多克隆抗体to NDC1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 323-372 (PPIIKYLALQ DLMLLSQYSP SRRQEVFSLS QPGGHPHNWT AISRECLNLL) of Human NDC1, NP_060557.

  • 阳性对照
    • Fetal lung lysate

性能

应用

Our Abpromise guarantee covers the use of ab83876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75, >90 kDa (predicted molecular weight: 75 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性NDC1 is a component of the nuclear pore complex (NPC), which plays a key role in de novo assembly and insertion of NPC in the nuclear envelope. NDC1 is required for NPC and nuclear envelope assembly, possibly by forming a link between the nuclear envelope membrane and soluble nucleoporins, thereby anchoring the NPC in the membrane. NDC1 colocalizes with p62 (NUP62) on the nuclear envelope at the nuclear rim. The protein has 6 transmembrane domains and several putative phosphorylation sites.
  • 细胞定位Nucleus; nuclear pore complex; multipass membrane protein
  • 数据库链接
  • 别名
    • FLJ10407 antibody
    • FLJ12556 antibody
    • FLJ34120 antibody
    • hNDC1 antibody
    • TMEM48 antibody
    • Transmembrane protein 48 antibody
    see all

Anti-NDC1 antibody 图像

  • Anti-NDC1 antibody (ab83876) at 1 µg/ml + fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 75 kDa
    Observed band size : 75,>90 kDa (why is the actual band size different from the predicted?)

Anti-NDC1 antibody (ab83876)参考文献

This product has been referenced in:
  • Tarazón E  et al. Heart failure induces significant changes in nuclear pore complex of human cardiomyocytes. PLoS One 7:e48957 (2012). WB ; Human . Read more (PubMed: 23152829) »

See 1 Publication for this product

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