Anti-NCR1抗体[MM0491-8F24] (ab89877)

概述

  • 产品名称Anti-NCR1抗体[MM0491-8F24]
    参阅全部 NCR1 一抗
  • 描述
    小鼠单克隆抗体[MM0491-8F24] to NCR1
  • 经测试应用适用于: WB, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to the extracellular domain of Human NCR1.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and sterile filtered through a 0.2 µm filter.
  • 克隆单克隆
  • 克隆编号MM0491-8F24
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab89877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
IHC-Fr 1/20 - 1/100.
Flow Cyt 1/50 - 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
  • 组织特异性Selectively expressed by both resting and activated NK cells.
  • 序列相似性Belongs to the natural cytotoxicity receptor (NCR) family.
    Contains 2 Ig-like (immunoglobulin-like) domains.
  • 翻译后修饰N-glycosylated.
    O-glycosylated.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating NK receptor NK p46 antibody
    • CD335 antibody
    • FLJ99094 antibody
    • hNKp46 antibody
    • Ly94 antibody
    • Lymphocyte antigen 94 antibody
    • Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) antibody
    • Lymphocyte antigen 94 homolog antibody
    • Natural cytotoxicity triggering receptor 1 antibody
    • Natural killer cell p46-related protein antibody
    • NCR1 antibody
    • NCT1 antibody
    • NCTR1_HUMAN antibody
    • NK cell activating receptor antibody
    • NK cell-activating receptor antibody
    • NK-p46 antibody
    • NKp46 antibody
    see all

Anti-NCR1 antibody [MM0491-8F24] (ab89877)参考文献

ab89877 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (peripheral blood mononuclear cells (PBMC))
Permeabilization No
Gating Strategy lymphocytes based on SSC vs FSC
Specification peripheral blood mononuclear cells (PBMC)
Fixation Paraformaldehyde
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提交于 Oct 14 2016

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Osteosarcoma)
Permeabilization No
Specification Osteosarcoma
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative Formaldehyde
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提交于 Jun 25 2015

Application Flow Cytometry
Sample Human Cell (PBMCs)
Permeabilization No
Gating Strategy Live cell and PBMC population
Specification PBMCs
Fixation Formaldehyde
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提交于 Jun 16 2015

Application Western blot
Sample Human Cell lysate - whole cell (NK cells)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification NK cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
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提交于 Jun 02 2015

Thank you for contacting Abcam.

For ab89877, the isotype is IgG2b.

If there is anything else I can help you with, please let me know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"