Anti-NCR1抗体[MM0491-8F24] (ab89877)

概述

  • 产品名称
    Anti-NCR1抗体[MM0491-8F24]
    参阅全部 NCR1 一抗
  • 描述
    小鼠单克隆抗体[MM0491-8F24] to NCR1
  • 经测试应用
    适用于: WB, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to the extracellular domain of Human NCR1.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and sterile filtered through a 0.2 µm filter.
  • 克隆
    单克隆
  • 克隆编号
    MM0491-8F24
  • 同种型
    IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab89877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
IHC-Fr 1/20 - 1/100.
Flow Cyt 1/50 - 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
  • 组织特异性
    Selectively expressed by both resting and activated NK cells.
  • 序列相似性
    Belongs to the natural cytotoxicity receptor (NCR) family.
    Contains 2 Ig-like (immunoglobulin-like) domains.
  • 翻译后修饰
    N-glycosylated.
    O-glycosylated.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating NK receptor NK p46 antibody
    • CD335 antibody
    • FLJ99094 antibody
    • hNKp46 antibody
    • Ly94 antibody
    • Lymphocyte antigen 94 antibody
    • Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) antibody
    • Lymphocyte antigen 94 homolog antibody
    • Natural cytotoxicity triggering receptor 1 antibody
    • Natural killer cell p46-related protein antibody
    • NCR1 antibody
    • NCT1 antibody
    • NCTR1_HUMAN antibody
    • NK cell activating receptor antibody
    • NK cell-activating receptor antibody
    • NK-p46 antibody
    • NKp46 antibody
    see all

Anti-NCR1 antibody [MM0491-8F24] (ab89877)参考文献

ab89877 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (peripheral blood mononuclear cells (PBMC))
Permeabilization
No
Gating Strategy
lymphocytes based on SSC vs FSC
Specification
peripheral blood mononuclear cells (PBMC)
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 14 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Osteosarcoma)
Permeabilization
No
Specification
Osteosarcoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 25 2015

Application
Flow Cytometry
Sample
Human Cell (PBMCs)
Permeabilization
No
Gating Strategy
Live cell and PBMC population
Specification
PBMCs
Fixation
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 16 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (NK cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
NK cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

提交于 Jun 02 2015

Thank you for contacting Abcam.

For ab89877, the isotype is IgG2b.

If there is anything else I can help you with, please let me know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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