Anti-NCOR2抗体- ChIP Grade (ab5802)

概述

  • 产品名称Anti-NCOR2抗体- ChIP Grade
    参阅全部 NCOR2 一抗
  • 描述
    兔多克隆抗体to NCOR2 - ChIP Grade
  • 特异性ab5802 detects silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) from human cells.
  • 经测试应用适用于: WB, ICC, ICC/IF, IP, ChIP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human, Drosophila melanogaster
  • 免疫原

    Fusion protein corresponding to Human NCOR2 aa 95-565.

  • 阳性对照
    • HeLa cell nuclear extracts.

性能

应用

Our Abpromise guarantee covers the use of ab5802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 4 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 302 kDa).
ICC 1/100 - 1/200.
ICC/IF 1/100 - 1/200.
IP Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
IHC-P 1/100 - 1/1000.

靶标

  • 功能Transcriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state (By similarity). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors.
  • 组织特异性Ubiquitous. High levels of expression are detected in lung, spleen and brain.
  • 序列相似性Belongs to the N-CoR nuclear receptor corepressors family.
    Contains 2 SANT domains.
  • 结构域The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).
    The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • CTG 26 antibody
    • CTG repeat protein 26 antibody
    • CTG26 antibody
    • N CoR2 antibody
    • N-CoR2 antibody
    • NCoR 2 antibody
    • Ncor2 antibody
    • NCOR2_HUMAN antibody
    • Nuclear Receptor Co-Repressor 2 antibody
    • Nuclear receptor corepressor 2 antibody
    • retinoic-acid-receptor-associated corepressor antibody
    • Silencing Mediator for Retanoid and Thyroid Hormone Receptors antibody
    • Silencing mediator of retinoic acid and thyroid hormone receptor antibody
    • SMAP 270 antibody
    • SMAP270 antibody
    • SMRT antibody
    • SMRTE antibody
    • SMRTE tau antibody
    • T3 receptor associating factor antibody
    • T3 receptor-associating factor antibody
    • Thyroid retinoic acid receptor associated corepressor antibody
    • Thyroid- antibody
    • TNRC 14 antibody
    • TNRC14 antibody
    • TRAC 1 antibody
    • TRAC antibody
    • TRAC1 antibody
    see all

Anti-NCOR2 antibody - ChIP Grade 图像

  • Immunofluorescent analysis of NCOR2 (green) showing staining in the nucleus of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunofluorescent analysis of NCOR2 (green) showing staining in the nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunofluorescent analysis of NCOR2 (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1:150 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.

  • Immunofluorescent analysis of NCOR2 (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab5802 in 3% BSA-PBS at a dilution of 1:150 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.

  • ab5802 labelling NCoR2 in mouse brain tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). Antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min and tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with primary antibody (1:500 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab5802 labelling NCoR2 in human lung tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). Antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min and tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with primary antibody (1:500 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Anti-NCOR2 antibody - ChIP Grade (ab5802)参考文献

ab5802 has not yet been referenced specifically in any publications.

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