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In ChIP ab2780 gives a maximum at 60 min (more than 60%) whereas ab2781 gives a minimum (around 5%) at this time and ab 2780 gives a minimum (less than 5%) at 30 min whereas ab2781 gives a maximum (50%). This protein is corepressor of the nuclear hormone receptors and is not supposed to be recruited in presence of the hormone (here oestrogen), and should be maximal before induction, which is not the case here for both antibodies!
Our Abpromise guarantee covers the use of ab2781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 4 µg/ml. Detects a band of approximately 250, 220 kDa (predicted molecular weight: 274 kDa).Can be blocked with Retinoic Acid Receptor alpha peptide (ab4910).|
|Flow Cyt||Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|