Anti-NCOA62抗体(ab70827)

概述

  • 产品名称Anti-NCOA62抗体
    参阅全部 NCOA62 一抗
  • 描述
    兔多克隆抗体to NCOA62
  • 经测试应用适用于: IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 450 and 500 of human NCOA62 (NP_036377.1).

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab70827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性Nuclear receptor coactivator NCoA62 (Nuclear Protein SkiP, SKIIP , ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • Bx 42 antibody
    • Bx42 antibody
    • Homolog of Drosophila BX42 antibody
    • MGC119379 antibody
    • NCOA 62 antibody
    • Nuclear protein SkiP antibody
    • Nuclear receptor coactivator 62 kD antibody
    • Nuclear receptor coactivator NCoA 62 antibody
    • Nuclear receptor coactivator NCoA62 antibody
    • Prp 45 antibody
    • Prp45 antibody
    • PRPF 45 antibody
    • PRPF45 antibody
    • Ski interacting protein antibody
    • SKIIP antibody
    • SKIP antibody
    • SNW 1 antibody
    • SNW domain containing 1 antibody
    • SNW domain containing protein 1 antibody
    • SNW1 antibody
    • SNW1 protein antibody
    see all

Anti-NCOA62 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling NCOA62 with ab70827 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NCOA62 antibody (ab70827) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 59 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,40 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human NCOA62 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70827 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

Anti-NCOA62 antibody (ab70827)参考文献

ab70827 has not yet been referenced specifically in any publications.

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Do you have any preliminary data regarding the sequence homology between Drosophila and human NCOA62?

According to the Blast search, the homology with Xenopus laevis ( Am...

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