概述

  • 产品名称Anti-NCOA5抗体
    参阅全部 NCOA5 一抗
  • 描述
    兔多克隆抗体to NCOA5
  • 经测试应用适用于: IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    A region between residues 1 and 50 of human NCOA5 (NP_066018.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab70831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
WB 1/1000 - 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 相关性NCOA5 is a nuclear receptor coregulator that can have both coactivator and corepressor functions. It interacts with nuclear receptors for steroids (ESR1 and ESR2) independently of the steroid binding domain (AF2) of the ESR receptors, and with the orphan nuclear receptor NR1D2. It Is also involved in the coactivation of nuclear steroid receptors (ER) as well as the corepression of MYC/c myc in response to 17 beta estradiol (E2).
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • bA465L10.6 antibody
    • CIA antibody
    • Coactivator independent of AF 2 antibody
    • KIAA1637 antibody
    • MGC28864 antibody
    • NCoA 5 antibody
    • NCOA5 antibody
    • Nuclear receptor coactivator 5 antibody
    • OTTMUSP00000001057 antibody
    • RP23-61O3.13 antibody
    see all

Anti-NCOA5 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling NCOA5 with ab70831 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NCOA5 antibody (ab70831) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 3 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 66 kDa
    Observed band size : 66 kDa


    Exposure time : 3 minutes
  • Detection of NCOA5 by Western Blot of Immunprecipitate.
    ab70831 at 1µg/ml staining NCOA5 in HeLa whole cell lysate immunoprecipitated using ab70831 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-NCOA5 antibody (ab70831)参考文献

ab70831 has not yet been referenced specifically in any publications.

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