概述

  • 产品名称Anti-NCOA4抗体
    参阅全部 NCOA4 一抗
  • 描述
    小鼠单克隆抗体to NCOA4
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to the C terminal amino acids 505-614 (EGSPKEVPGT EDRAGKQKFK SPMNTSWCSF NTADWVLPGK KMGNLSQLSS GEDKWLLRKK AQEVLLNSPL QEEHNFPPDH YGLPAVCDLF ACMQLKVDKE KWLYRTPLQM) of human NCOA4 (NP_005428) linked to a proprietary tag.

  • 阳性对照
    • IHC-P: Human pancreas WB: The immunogen; HeLa nuclear extract

性能

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应用

Our Abpromise guarantee covers the use of ab89222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 70 kDa.
IHC-P Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.

靶标

  • 功能Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma.
  • 组织特异性Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis.
  • 疾病相关Defects in NCOA4 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving NCOA4 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 70 kDa androgen receptor activator antibody
    • 70 kDa androgen receptor coactivator antibody
    • 70 kDa AR activator antibody
    • 70 kDa AR-activator antibody
    • Androgen receptor coactivator 70 kD antibody
    • Androgen receptor coactivator 70 kDa protein antibody
    • Androgen receptor-associated protein of 70 kDa antibody
    • ARA 70 antibody
    • ARA70 antibody
    • DKFZp762E1112 antibody
    • ELE 1 antibody
    • ELE1 antibody
    • ELE1/ret TK antibody
    • NCOA 4 antibody
    • NCoA-4 antibody
    • NCOA4 antibody
    • NCOA4_HUMAN antibody
    • Nuclear receptor coactivator 4 antibody
    • Papillary thyroid carcinoma 3 antibody
    • PTC 3 antibody
    • PTC3 antibody
    • RET activating gene ELE1 antibody
    • Ret activating protein ELE1 antibody
    • Ret-activating protein ELE1 antibody
    • RFG antibody
    see all

Anti-NCOA4 antibody 图像

  • Anti-NCOA4 antibody (ab89222) at 5 µg/ml + immunogen at 0.2 µg
    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-NCOA4 antibody (ab89222) at 5 µg/ml + HeLa nuclear extract at 50 µg
    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : ~60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : ~13 kDa. We are unsure as to the identity of these extra bands.
  • ab89222, at 3 µg/ml, staining NCOA4 in formalin fixed, paraffin embedded human pancreas tissue by Immunoperoxidase /Immunohistochemistry.
  • ab89222, at 10 µg/ml, detecting recombinant tagged NCOA4 in a sandwich ELISA. The detection limit for recombinant tagged NCOA4 is approximately 0.3 ng/ml when using ab89222 as a capture antibody.

Anti-NCOA4 antibody (ab89222)参考文献

ab89222 has not yet been referenced specifically in any publications.

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