Anti-NCOA4抗体[439CT10.1.2] (ab62495)
Key features and details
- Mouse monoclonal [439CT10.1.2] to NCOA4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgM
概述
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产品名称
Anti-NCOA4抗体[439CT10.1.2]
参阅全部 NCOA4 一抗 -
描述
小鼠单克隆抗体[439CT10.1.2] to NCOA4 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human NCOA4 aa 400 to the C-terminus (C terminal).
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阳性对照
- This antibody gave a positive signal in HEK293 and PC3 whole cell lysates.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Concentrated Culture Supernatant -
克隆
单克隆 -
克隆编号
439CT10.1.2 -
骨髓瘤
Sp2/0 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62495于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/100. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa). Use 3% milk as blocking agent.
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说明 |
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WB
1/100. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa). Use 3% milk as blocking agent. |
靶标
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功能
Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma. -
组织特异性
Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis. -
疾病相关
Defects in NCOA4 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving NCOA4 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas. - Information by UniProt
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数据库链接
- Entrez Gene: 8031 Human
- Omim: 601984 Human
- SwissProt: Q13772 Human
- Unigene: 643658 Human
- Unigene: 709644 Human
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别名
- 70 kDa androgen receptor activator antibody
- 70 kDa androgen receptor coactivator antibody
- 70 kDa AR activator antibody
see all
图片
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All lanes : Anti-NCOA4 antibody [439CT10.1.2] (ab62495) at 1/100 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Abcam recommends using 3% milk as the blocking agent.
数据表及文件
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Datasheet download
文献 (1)
ab62495 被引用在 1 文献中.
- Luo H & Zhang R Icariin enhances cell survival in lipopolysaccharide-induced synoviocytes by suppressing ferroptosis via the Xc-/GPX4 axis. Exp Ther Med 21:72 (2021). PubMed: 33365072