概述

  • 产品名称Anti-NCKIPSD抗体
    参阅全部 NCKIPSD 一抗
  • 描述
    小鼠多克隆抗体to NCKIPSD
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length human NCKIPSD protein (NP_909119.1).

  • 阳性对照
    • Rat brain tissue lysate, HeLa cell lysate and NCKIPSD transfected 293T cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab88467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23024796

靶标

  • 功能Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion.
  • 组织特异性Highest expression in heart, brain, skeletal muscle, kidney and liver. Lower levels in placenta, lung, small intestine and leukocytes. Weak expression in colon, thymus and spleen.
  • 疾病相关Note=A chromosomal aberration involving NCKIPSD/AF3p21 is found in therapy-related leukemia. Translocation t(3;11)(p21;q23) with MLL.
  • 序列相似性Contains 1 SH3 domain.
  • 细胞定位Nucleus. Colocalizes with DRF1 at membrane ruffles, and with Nck at Z-disks in mature cardiac myocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 54 kDa VacA-interacting protein antibody
    • 54 kDa vimentin-interacting protein antibody
    • 90 kDa SH3 protein interacting with Nck antibody
    • AF3p21 antibody
    • dia interacting protein antibody
    • Dia-interacting protein 1 antibody
    • Diaphanous protein-interacting protein antibody
    • DIP antibody
    • DIP-1 antibody
    • DIP1 antibody
    • NCK interacting protein with SH3 domain antibody
    • NCK-interacting protein with SH3 domain antibody
    • Nckipsd antibody
    • ORF1 antibody
    • SH3 adapter protein SPIN90 antibody
    • SH3 protein interacting with Nck 90 kDa antibody
    • SPIN90 antibody
    • SPN90_HUMAN antibody
    • VIP54 antibody
    • WASLBP antibody
    • WASP-interacting SH3-domain protein antibody
    • WISH antibody
    • wiskott- Aldrich syndrome protein-interacting protein antibody
    • Wiskott-Aldrich syndrome protein-interacting protein antibody
    see all

Anti-NCKIPSD antibody 图像

  • Anti-NCKIPSD antibody (ab88467) at 1 µg/ml + rat brain tissue lysate at 50 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 79 kDa
    Observed band size : 79 kDa
  • Anti-NCKIPSD antibody (ab88467) at 1 µg/ml + HeLa cell lysate at 50 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 79 kDa
    Observed band size : 79 kDa
  • All lanes : Anti-NCKIPSD antibody (ab88467) at 1 µg/ml

    Lane 1 : NCKIPSD transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 79 kDa
    Observed band size : 79 kDa

Anti-NCKIPSD antibody (ab88467)参考文献

This product has been referenced in:
  • Wyse MM  et al. Dia-Interacting Protein (DIP) Imposes Migratory Plasticity in mDia2-Dependent Tumor Cells in Three-Dimensional Matrices. PLoS One 7:e45085 (2012). ICC/IF . Read more (PubMed: 23024796) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step None
Permeabilization Yes - 0.01%TritonX-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Paraformaldehyde
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Ben Siemsen

Verified customer

提交于 Jun 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"