Anti-NCKAP1L抗体(ab82664)

概述

  • 产品名称
    Anti-NCKAP1L抗体
    参阅全部 NCKAP1L 一抗
  • 描述
    兔多克隆抗体to NCKAP1L
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A synthetic peptide corresponding to a region within the C terminal amino acids 1008-1057 (LPLLATDPSS FYSIEKDGYN NNIHCLTKAI IQVSAALFTL YNKNIETHLK) of human NCKAP1L (NP_005328).

  • 阳性对照
    • 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab82664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 128 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

靶标

图片

  • Anti-NCKAP1L antibody (ab82664) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1/50000 - 1/100000 as secondary antibody.

    Predicted band size : 128 kDa
    Observed band size : 128 kDa

实验方案

文献

ab82664 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (both hematopoietic and adherent cell lines)
Loading amount
20 µg
Specification
both hematopoietic and adherent cell lines
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Jul 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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